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prestained protein ladders  (Bio-Rad)


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    Structured Review

    Bio-Rad prestained protein ladders
    Prestained Protein Ladders, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 97/100, based on 2717 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/color+prestained+protein+standard/Precision+Plus+Protein+Dual+Color+Standards/pmc13618816-343-55-58
    Average 97 stars, based on 2717 article reviews
    prestained protein ladders - by Bioz Stars, 2026-10
    97/100 stars

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    SDS Page:

    Article Title: Biochemical features of the novel Tail Tubular Protein A of Yersinia phage phiYeO3-12
    Article Snippet: .. Figure 3 Analysis of TTPAgp11 from Yersinia phage phiYeO3-12 using 10% ( A ) and 12.5% ( B ) SDS-PAGE. ( A ) The lanes are as follows: (1) Spectra Multicolor Broad Range Protein Ladder (Bio-Rad); (2) crude extract; (3) pooled fractions after the first round of Ni2+ -affinity chromatography; (4) protein solution after TEV protease cleavage. ( B ) The lanes are as follows: (1) Color Prestained Protein Standard, Broad Range (Bio-Rad); (2) pure target protein - TTPAgp11. ..

    Article Title: Intrinsic resistance to short-tailed azoles in the basal fungus Mucor lusitanicus : functional analysis of Cyp51 isoforms and amino acid substitutions
    Article Snippet: The protein content of the crude membrane fraction was determined with Bovine serum albumin (Thermo Fisher Scientific TM , Waltham, MA, United States) as standard. .. Samples of 20 μg of protein were separated by SDS-PAGE on 8% acrylamide gels according the method of and either stained with Coomassie blue R250 or electro-transferred onto nitrocellulose membranes (BioRad, Feldkirchen, GER) using the Trans-Blot Turbo Transfer System (BioRad, Feldkirchen, GER) according to a standard protocol at 25 V for 30 min. Color Prestained Protein Standard, Broad Range (10–250 kDa, BioRad, Feldkirchen, GER) served as a size standard for SDS PAGE and Western blots. .. His 6 -tagged proteins were decorated with a conjugated antibody (Anti-His 6 -peroxidase from mouse, Merck, Vienna, AUT) and antigen–antibody complexes detected by ECL using the ImageQuant TM LAS 4000 Imaging System (GE Healthcare, Tiefenbach, AUT) ( ).

    Affinity Chromatography:

    Article Title: Biochemical features of the novel Tail Tubular Protein A of Yersinia phage phiYeO3-12
    Article Snippet: .. Figure 3 Analysis of TTPAgp11 from Yersinia phage phiYeO3-12 using 10% ( A ) and 12.5% ( B ) SDS-PAGE. ( A ) The lanes are as follows: (1) Spectra Multicolor Broad Range Protein Ladder (Bio-Rad); (2) crude extract; (3) pooled fractions after the first round of Ni2+ -affinity chromatography; (4) protein solution after TEV protease cleavage. ( B ) The lanes are as follows: (1) Color Prestained Protein Standard, Broad Range (Bio-Rad); (2) pure target protein - TTPAgp11. ..

    other:

    Article Title: Compartmentalization of the SUMO/RNF4 pathway by SLX4 drives DNA repair.
    Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Deposited Data Raw data This paper Mendeley Dataset: https://doi.org/10.17632/c5h63ykhpn.1 Experimental Models: Cell Lines Flp-In T-REx 293 Invitrogen Cat# R78007; RRID:CVCL_U427 SLX4-null (RA3331/E6E7/hTERT) Gift from Dr. Agata Smogorzewska N/A SLX4-complemented (RA3331/E6E7/hTERT) Gift from Dr. Agata Smogorzewska N/A HEK293T Sigma-Aldrich Cat# 12022001; RRID:CVCL_0063 CL-HJL-0104-A KI HEK 293T Biocytogen N/A Oligonucleotides siGENOME Non-Targeting siRNA Control Pools Dharmacon_Horizon Discovery Cat# ID:D-001206-13-20 siGENOME Human SLX4 siRNA Dharmacon_Horizon Discovery Cat# ID:M-014895-01-0020 siGENOME Human RNF4 siRNA Dharmacon_Horizon Discovery Cat# ID:M-006557-03-0020 siGENOME Human UBE2I siRNA Dharmacon_Horizon Discovery Cat# ID:M-004910-00-0020 siGENOME Human SENP6 siRNA Dharmacon_Horizon Discovery Cat# ID:M-006044-01-0020 The oligonucleotides used in this study are listed in Table S2 N/A Recombinant DNA pCDNA5_FRT_TO_TurboID-mCherry-Cry2 Addgene Cat# 166504; http://n2t.net/addgene:166504; RRID:Addgene_166504 pOG44 Flp-Recombinase Expression Vector ThermoFisher Scientific Cat# V600520 pLenti-CMV-Blast-PIP-FUCCI Addgene Cat# 138715; http://n2t.net/addgene:138715; RRID:Addgene_138715 pCRISPaint-HaloTag-PuroR Addgene Cat# 80960; http://n2t.net/addgene:80960; RRID:Addgene_80960 Software and Algorithms OMERO OME Remote Objects software RRID:SCR_002629 Image Lab Software (Version 5.2.1) Bio-Rad RRID:SCR_014210 Biorender Software Science Suite Inc. RRID:SCR_018361 Prism 8 (Version 8.4.2) Graphpad Software RRID:SCR_002798 FIJI Open source RRID:SCR_002285 Xcalibur (Version 4.0) Thermo Scientific RRID:SCR_014593 MaxQuant (Version 1.6.10.43) Max-Planck-Institute of Biochemistry RRID:SCR_014485 Other CO2 Incubator C150 Binder Cat# 9040-0078 Sonicator, VibraCell- 72405 BioBlock scientific N/A KNF LABOPORT Mini Diaphragm Vacuum Pump N 811 in Pumps, Compressors Dominique Dutscher Cat# KNF_28002 Centrifuge Hettich Mikro 200 Grosseron N/A Mini-PROTEAN Tetra Vertical Electrophoresis Cell Bio-Rad Cat# 1658004 PowerPac HC High-Current Power Supply Bio-Rad Cat# 1645052 Trans-Blot Turbo Transfer System Bio-Rad Cat# 1704150 Slot Blot blotting manifold Hoefer Cat# PR648 Chemical Duty Pump, 220 V/50 Hz Merck Cat# WP6122050 Acclaim PepMap 100 C18 HPLC Columns ThermoFisher Scientific Cat# 164568 e4 Molecular Cell 83, 1640–1658.e1–e9, May 18, 2023

    Article Title: Spatial organization and functions of Chk1 activation by TopBP1 biomolecular condensates.
    Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER pCRISPaint-HaloTag-PuroR Addgene Cat# 80960; http://n2t.net/addgene:80960; RRID:Addgene_80960 pCDNA5_FRT-TO_TurboID-TopBP1WTmCherry-Cry2 Frattini et al., molcel (2021)23 N/A pCDNA5_FRT-TO_TurboID-TopBP1S1273AmCherry-Cry2 This paper N/A pCDNA5_FRT-TO_TurboID-TopBP1R1280QmCherry-Cry2 This paper N/A pCDNA5_FRT-TO_TurboID-TopBP1R1314QmCherry-Cry2 This paper N/A pCDNA5_FRT-TO_TurboID-TopBP1M1mCherry-Cry2 This paper N/A pCDNA5_FRT-TO_TurboID-TopBP1M2mCherry-Cry2 This paper N/A pCDNA5_FRT-TO_TurboID-TopBP1M3mCherry-Cry2 This paper N/A pCDNA5_FRT-TO_TurboID-TopBP1M4mCherry-Cry2 This paper N/A pCDNA5_FRT-TO_TurboID-TopBP1M5mCherry-Cry2 This paper N/A pCDNA5_FRT-TO_TurboID-TopBP1M3RmCherry-Cry2 This paper N/A Software and algorithms OMERO OME Remote Objects software RRID:SCR_002629 Image LabTM Software (Version 5.2.1) Bio-Rad RRID:SCR_014210 Biorender Software Science Suite Inc. RRID:SCR_018361 Prism 8 (Version 8.4.2) Graphpad Software RRID:SCR_002798 FIJI Open source RRID:SCR_002285 Kaluza Aquisition Software Beckman Coulter RRID:SCR_016182 Kaluza Analysis Software Beckman Coulter RRID:SCR_016182 Cell Profiler 4.2.1 CellProfiler Image Analysis Software RRID:SCR_007358 ZEN Blue Ziess RRID:SCR_013672 Other CO2 Incubator C150 Binder Cat# 9040-0078 Sonicator, VibraCell- 72405 BioBlock scientific N/A Centrifuge Hettich Mikro 200 Grosseron N/A CriterionTM Cell Bio-Rad Cat# 1656001 PowerPacTM HC High-Current Power Supply Bio-Rad Cat# 1645052 Trans-Blot Turbo Transfer System Bio-Rad Cat# 1704150 Gallios Flow Cytometer Beckman Coulter Gallios Flow Cytometer RRID:SCR_019639 Zen Axio Imager 2 Zeiss RRID:SCR_018876

    Staining:

    Article Title: Intrinsic resistance to short-tailed azoles in the basal fungus Mucor lusitanicus : functional analysis of Cyp51 isoforms and amino acid substitutions
    Article Snippet: The protein content of the crude membrane fraction was determined with Bovine serum albumin (Thermo Fisher Scientific TM , Waltham, MA, United States) as standard. .. Samples of 20 μg of protein were separated by SDS-PAGE on 8% acrylamide gels according the method of and either stained with Coomassie blue R250 or electro-transferred onto nitrocellulose membranes (BioRad, Feldkirchen, GER) using the Trans-Blot Turbo Transfer System (BioRad, Feldkirchen, GER) according to a standard protocol at 25 V for 30 min. Color Prestained Protein Standard, Broad Range (10–250 kDa, BioRad, Feldkirchen, GER) served as a size standard for SDS PAGE and Western blots. .. His 6 -tagged proteins were decorated with a conjugated antibody (Anti-His 6 -peroxidase from mouse, Merck, Vienna, AUT) and antigen–antibody complexes detected by ECL using the ImageQuant TM LAS 4000 Imaging System (GE Healthcare, Tiefenbach, AUT) ( ).

    Western Blot:

    Article Title: Intrinsic resistance to short-tailed azoles in the basal fungus Mucor lusitanicus : functional analysis of Cyp51 isoforms and amino acid substitutions
    Article Snippet: The protein content of the crude membrane fraction was determined with Bovine serum albumin (Thermo Fisher Scientific TM , Waltham, MA, United States) as standard. .. Samples of 20 μg of protein were separated by SDS-PAGE on 8% acrylamide gels according the method of and either stained with Coomassie blue R250 or electro-transferred onto nitrocellulose membranes (BioRad, Feldkirchen, GER) using the Trans-Blot Turbo Transfer System (BioRad, Feldkirchen, GER) according to a standard protocol at 25 V for 30 min. Color Prestained Protein Standard, Broad Range (10–250 kDa, BioRad, Feldkirchen, GER) served as a size standard for SDS PAGE and Western blots. .. His 6 -tagged proteins were decorated with a conjugated antibody (Anti-His 6 -peroxidase from mouse, Merck, Vienna, AUT) and antigen–antibody complexes detected by ECL using the ImageQuant TM LAS 4000 Imaging System (GE Healthcare, Tiefenbach, AUT) ( ).



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